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The blot displays Cyclin B1 levels in lysates made from either an asynchronous cell population (Async) or cells treated with 100 ng/mL nocodazole for 16 h and harvested via mitotic shake-off (Mitotic). <t>α</t> <t>Tubulin</t> and Histone H3 are included as loading controls. This representative image was obtained from one biological repeat.
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The blot displays Cyclin B1 levels in lysates made from either an asynchronous cell population (Async) or cells treated with 100 ng/mL nocodazole for 16 h and harvested via mitotic shake-off (Mitotic). <t>α</t> <t>Tubulin</t> and Histone H3 are included as loading controls. This representative image was obtained from one biological repeat.
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The blot displays Cyclin B1 levels in lysates made from either an asynchronous cell population (Async) or cells treated with 100 ng/mL nocodazole for 16 h and harvested via mitotic shake-off (Mitotic). <t>α</t> <t>Tubulin</t> and Histone H3 are included as loading controls. This representative image was obtained from one biological repeat.
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The blot displays Cyclin B1 levels in lysates made from either an asynchronous cell population (Async) or cells treated with 100 ng/mL nocodazole for 16 h and harvested via mitotic shake-off (Mitotic). <t>α</t> <t>Tubulin</t> and Histone H3 are included as loading controls. This representative image was obtained from one biological repeat.
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The blot displays Cyclin B1 levels in lysates made from either an asynchronous cell population (Async) or cells treated with 100 ng/mL nocodazole for 16 h and harvested via mitotic shake-off (Mitotic). <t>α</t> <t>Tubulin</t> and Histone H3 are included as loading controls. This representative image was obtained from one biological repeat.
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The blot displays Cyclin B1 levels in lysates made from either an asynchronous cell population (Async) or cells treated with 100 ng/mL nocodazole for 16 h and harvested via mitotic shake-off (Mitotic). α Tubulin and Histone H3 are included as loading controls. This representative image was obtained from one biological repeat.

Journal: Bio-protocol

Article Title: Denaturing SUMO Immunoprecipitation From Mitotic Cells

doi: 10.21769/BioProtoc.5648

Figure Lengend Snippet: The blot displays Cyclin B1 levels in lysates made from either an asynchronous cell population (Async) or cells treated with 100 ng/mL nocodazole for 16 h and harvested via mitotic shake-off (Mitotic). α Tubulin and Histone H3 are included as loading controls. This representative image was obtained from one biological repeat.

Article Snippet: RANGAP1 polyclonal antibody (Invitrogen, catalog number: PA5-92435) 39. (Optional) Pierce TM 660 nm protein assay (ThermoFisher Scientific, catalog number: 22660) 40. (Optional) ionic detergent compatibility reagent (ThermoFisher Scientific, catalog number: 22663) 41. (Optional) Cyclin B1 antibody (Cell Signaling, catalog number: 12231) 42. (Optional) α Tubulin antibody (Novus Biologicals, catalog number: NB100-690) 43. (Optional) Histone H3 (Cell Signaling, catalog number: 9715) Solutions 1.

Techniques: